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KMID : 0371319960500010015
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Journal of the Korean Surgical Society
1996 Volume.50 No. 1 p.15 ~ p.19
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The Application of Splenoportography on the Study Using Portacaval Shunt in the Rat
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유창현
신정국/김준현/김병호
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Abstract
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The portacaval shunt(PCS) procedure is frequently employed for studying the effects of portal-systemic shunting of splanchnic blood. In many species, a PCS eventually leads to hepatic failure and death, but the rat has been known to survive and
regain
weight. Some investigators explained the mechanism of weight-sparing effect as due to portal hypertension from stenosis of PCS which induces hepatopedal collaterals which in turn prevent atrophy. This also may explain the different results
obtained
between investigators. Some recent studies using the shunt in the rat closed their eye to the necessity to confirm that the shunt was opened by splenoportography. We studied two groups of Sprague-Dawley rats. In Group I, the shunt stomal size was
maximal(n=10); in group II, the stoma was constricted to guarter by a ligature at the first week of shunt operation (n=10). The serum enzyme activities of AST, alkaline phosphatase and r-GTP(Glutamyl transpeptidase) and bilirubin were normalized
within
2 weeks in bothe groups. Body weight decreased for 10 days after surgery and then increased at significantly different rates between the two groups. Weight-gain of group II was significantly faster than group I because the collaterals prevented
the
liver of group II form the severe atrophy noted in group I(p<0.05). Splenoportographies were taken at the 4th and 6th week post-surgery. All rats of group I showed an open-shunt without stomal stenosis. All rats of group II at the 6th week showed
the
collaterals with visualization of liver. In this study, we stress the importance of perfect microsurgical technique and the need for splenoportography to confirm the shunt open.
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KEYWORD
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